AGE RELATED BALDNESS Table I Composition of Preparations Used in Treatment Regimen 487 A. Conditioner 2-propanol 50 water 45 methyl ethyl ketone 4 Tween 80 0.39 smaller quantities of dye, perfume, allantoin, hexachlorophene and benzethonium chloride B. Cleanser inert oil 60 sulfonated vegetable oil 40 C. Shampoo water 58 ammonium lauryl sulfate 38.4 lauric diethanolamide 0.96 propylene glycol 0.96 smaller quantities of dichlorophene, methyl paraben, BHT, EDTA, dye and perfume D. Lotion 2-propanol 88 water 9.8 polyethylene glycol 400 2.1 smaller quantities of hexachlorophene and estradiol NF (the latter at a level of 0.00004 per cent or 0.0 ! 1 mg/fluid oz) E. Antiseptic dressing water 99.8 benzethonium chloride 0.19 smaller quantities of perfume and dye outcomes less favorable than any patient who completed the study, and are so included in the tables. MEDICATIONS During the control period, all subjects shampooed once every 3 to 7 days, using a mild unmedicated shampoo. During the treatment phase each subject was advised to apply in sequence each day the 5 liquid preparations whose compositions are given in Table I. PROCEDURE At the first interview each patient received a complete explanation of the protocol and signed a statement indicating informed consent. During an initial control period of 2 months we obtained 3 or more 24 h hair collections counted in an envelope. At each visit the examiner regraded the subject's signs and symptoms. A 1.5 x 1.5 cm site near the crown was clipped and photographed. The same area was located and reclipped at each return visit. The photos were later analyzed by applying a transparent 1 x 1 cm mask and visually enumerating hairs found growing within its area. Approximately 20 to 30 hairs were then plucked and separated into anagen and telogen categories under a hand lens (26). Next, a corneocyte count was made of squames removable from a 5.1 cm 2 area of the anterior forehead adjacent to the hair line (27).

488 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS Table II Summary of Results in 29 Subjects Who Met 2 Conditions: 1) Initial Rate of Hair Loss Equaled or Exceeded 50 Hairs/Day 2) Satisfactorily Completed a 2-Months Control Period and at Least 3 Months Treatment Period Women Men Total Reduction in hair loss 3/10 10/19 13/29 (45 per cent) ( 20 per cent) Increase in hairs/cm 2 5/8 1/13 6/21 (29 per cent) ( 20 per cent) a Subjective increase in hair 7/10 11/19 18/29 (62 per cent) aPhotographic counts of hair density were complete for only 8 women and 13 men, while compiete measure- ments of daily loss constituted a criterion for inclusion in the study. Table III Reduction in Hair Loss: Men Mean Daily Rate of Loss a Decrease as Signed Subject Control Period Treatment Period Difference Per Cent Control t' Rank RW 114 39 75 66 16 JP 133 43 90 68 17 MG 109 92 17 16 4.5 EG 96 173 -77 -80 -19 GT 192 78 114 59 15 RB 210 107 103 49 14 EF 86 48 38 44 13 JHo 277 68 209 75 18 FE 89 54 35 39 12 JW 107 113 -6 -6 -2 RD 197 131 66 34 10 WL-C 58 47 11 19 7 RP 93 108 -15 -16 -4.5 HG 80 96 -16 -20 -8 JM 59 62 -3 -5 -1 GW 63 42 21 33 9 JHa 162 191 -29 -18 -6 DC 74 81 -7 -9 -3 DG 200 131 69 35 11 RD • 99 c c (_81)• -20 TB e 105 e c (-82) c -21 n=21 5 Ri+ = 146.5 5 Ri-= 84.5 Wilcoxon Signed Rank Test: 0.067 p 0.081 •'Each value represents the mean of six determinations, 3 from each of 2 visits during control, and the same during treatment. bNegative signs in this and the difference column refer to rises in number of hairs lost. •These 2 patients dropped out after completing the control period, but before completing the treatment pe- riod. They were arbitrarily assigned the most unfavorable results on the table. At the end of the 2-month control period, each subject began using a large square of flexible plastic screen folded over the drain during the daily shampoo in order to collect lost hair. In addition to prior procedures the subject was questioned closely at