2006 TRI/PRINCETON CONFERENCE 377 of hydrophobic substitution is low, providing hydrophobic character similar to that of the PQ-10 polymer (UCARE™ LR-30M). Solutions of those polymers in water at 1 wt % level have viscosities of 2500-2800 cP (4). Ionic surfactants ALSCOAP* NS-230 [sodium lauryl ether (laureth) sulfate, 2 mol SLES-2 mol H(CH 2 ) 12 -(OCH 2 CH 2 ) 2 -O-SO 3 - Na+}, ALSCOAP* TH-330 [sodium lau- ryl ether sulfate, 3 mol SLES-3mol H(CH 2 ) 12 -(OCH 2 CH2) 3 -O-SO 3 - Na + }, OBAZO- RINE* CAB-30 (cocoamido-propyl betaine CAPB H(CH 2 ) 0 -CO-NH-(CH 2 ) 3 - N + (CH:)rCHrCO 2 -), NEOSCOAP* CN-30-SF (methylcocoyltaurate-desalinated), OBAZORINE* 662N (sodium cocoamphoacetate-non-desalinated), and OBAZO- RINE* 662N-SF (sodium cocoamphoacetate-desalinated) were obtained from TOHO Chemical Industry Co., Ltd. METHODS A Tecan 200 liquid handler (Tecan Systems, Inc.) was used to make formulations in 96-well micro-titer plates. The Tecan is fitted with a robotic arm with eight syringe tips, and each pipette is controlled by an individual syringe drive. Haze values were measured using a Nepheloskan Ascent (Thermo Labsystems) with an integration time of 20 ms, and the output data were in relative nephelometer units (RNU). The light scattering of each cell in the microtiter plate was corrected by subtra�ting the back- ground reading for an empty plate. For 3D visualization of data, Miner 3D software from Tableau Software was used. Preparation of formulations. Each formulation was prepared with one of the SL polymers, one of the SLES surfactants (2 mol or 3 mol), CAPB, and optionally with one of the remaining surfactants labeled as "surfactant 3" (CN-30-SF, 662N-Sf, or 662N). In formulations with the highest surfactant + polymer concentration (at dilution 1) the level of polymer was either 0.20 wt % or 0.40 wt %, the level of SLES was 6.00 wt %, 8.00 wt%, or 10.00 wt%, the level of CAPB was from 1.25 wt% to 5.00 wt%, while the level of "surfactant 3" was from Oto 3.75 wt %. The maximum level of CAPB and "surfactant 3" was limited to 5.00 wt%, while the total level of surfactants was limited to 15.00 wt%. Sixteen "surfactant packages" with different combinations of surfactants were chosen for this study (Table I). In our high throughput workflow water was placed in a source trough, while polymer and surfactant solutions were placed in mother plates. Those components were added to destination plates (in 8x8 arrangement) to form formulations with the desired compo- sition. Figure 2 shows schematically the composition of 64 formulations in a destination plate prepared at dilution 1. All formulations were prepared in 800 mg amounts. In the preparation of formulations shown in the Figure 2, 160 mg of 1.0 wt % aqueous solution of SL-5 was added into eight vials in column 1, and therefore final formulations had 0.2 wt % of this polymer. The same amount of 1.0 wt % solutions of polymers SL-30, SL-60, and SL-100 was added into the vials in columns 2, 3, and 4, respectively. The same procedure was repeated for the vials in columns 5 through 8, but in this case 320 mg of 1.0 wt % * Trademark of TOHO Chemical Industry Co., Ltd.

378 JOURNAL OF COSMETIC SCIENCE Table I Surfactant Levels in Formulations at Highest Concentration (Dilution 1) The surfactant level (wt %) Surf packa SLES6 CAPB s3c CAPB+S3 10.00 5.00 0.00 5.00 2 10.00 3.75 1.25 5.00 3 10.00 2.50 1.25 3.75 4 10.00 2.50 0.00 2.50 5 10.00 1.25 1.25 2.50 6 10.00 1.25 0.00 1.25 7 8.00 5.00 0.00 5.00 8 8.00 3.75 1.25 5.00 9 8.00 2.50 2.50 5.00 10 8.00 1.25 3.75 5.00 11 8.00 3.75 0.00 3.75 12 8.00 2.50 1.25 3.75 13 6.00 5.00 0.00 5.00 14 6.00 3.75 1.25 5.00 15 6.00 2.50 2.5 5.00 16 6.00 1.25 3.75 5.00 a Surfactant packages. 6 SLES-2 mol or SLES-3 mol. c The "surfactant 3" (CN-30-SF, 662N-Sf, or 662N). 4 6 7 A SL-5, 0.2% SL-30, 0.2% SL-60, 0.2% SL-100, 0.2% SL-5, 0.4% SL-30, 0.4% SL-60, 0.4% Surf Pack 1 Surf Pack 1 Surf Pack 1 Surf Pack 1 Surf Pack 1 Surf Pack 1 Surf Pack 1 B SL-5, 0.2% SL-30, 0.2% SL-60, 0.2% SL-100, 0.2% SL-5, 0.4% SL-30, 0.4% SL-60, 0.4% Surf Pack 2 Surf Pack 2 Surf Pack 2 Surf Pack 2 Surf Pack 2 Surf Pack 2 Surf Pack 2 C SL-5, 0.2% SL-30, 0.2% SL-60, 0.2% SL-100, 0.2% SL-5, 0.4% SL-30, 0.4% SL-60, 0.4% Surf Pack 3 Surf Pack 3 Surf Pack 3 Surf Pack 3 Surf Pack 3 Surf Pack 3 Surf Pack 3 D SL-5, 0.2% SL-30, 0.2% SL-60, 0.2% SL-100, 0.2% SL-5, 0.4% SL-30, 0.4% SL-60, 0.4% Surf Pack 4 Surf Pack 4 Surf Pack 4 Surf Pack 4 Surf Pack 4 Surf Pack 4 Surf Pack 4 SL-5, 0.2% SL-30, 0.2% SL-60, 0.2% SL-100, 0.2% SL-5, 0.4% SL-30, 0.4% SL-60, 0.4% Surf Pack 5 Surf Pack 5 Surf Pack 5 Surf Pack 5 Surf Pack 5 Surf Pack 5 Surf Pack 5 SL-5, 0.2% SL-30, 0.2% SL-60, 0.2% SL-100, 0.2% SL-5, 0.4% SL-30, 0.4% SL-60, 0.4% Surf Pack 6 Surf Pack 6 Surf Pack 6 Surf Pack 6 Surf Pack 6 Surf Pack 6 Surf Pack 6 G SL-5, 0.2% SL-30, 0.2% SL-60, 0.2% SL-100, 0.2% SL-5, 0.4% SL-30, 0.4% SL-60, 0.4% Surf Pack 7 Surf Pack 7 Surf Pack 7 Surf Pack 7 Surf Pack 7 Surf Pack 7 Surf Pack 7 H SL-5, 0.2% SL-30, 0.2% SL-60, 0.2% SL-100, 0.2% SL-5, 0.4% SL-30, 0.4% SL-60, 0.4% Surf Pack 8 Surf Pack 8 Surf Pack 8 Surf Pack 8 Surf Pack 8 Surf Pack 8 Surf Pack 8 Total 15.00 15.00 13.75 12.50 12.50 11.25 13.00 13.00 13.00 13.00 11.75 11.75 11.00 11.00 11.00 11.00 SL-100, 0.4% Surf Pack 1 SL-100, 0.4% Surf Pack 2 SL-100, 0.4% Surf Packi 3 SL-100, 0.4% Surf Pack 4 SL-100, 0.4% Surf Pack 5 SL-100, 0.4% Surf Pack 6 SL-100, 0.4% Surf Pack 7 SL-100, 0.4% Surf Pack 8 Figure 2. Schematic representation of a destination plate with formulations at dilution 1. aqueous solution of SL polymer was added, and final formulations in those vials had 0.4 wt % of polymer. Surfactants were added into eight vials in the row A in amounts that gave formulations with final concentrations required for surfactant package 1 (10.0 wt